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工业毕赤酵母被转换为利用CO2生长的自养型生物

来源:澳门金沙网站 |最近更新: 2019-12-18

本期文章:《自然—生物技术》:Online/在线发表

工业毕赤酵母由异养型转化为能够利用CO2生长的自养型生物,这一成果由奥地利自然资源与生命科学大学Diethard Mattanovich小组近期取得。这一研究成果2019年12月16日在线发表在国际学术期刊《自然—生物技术》上。

为朝着更具可持续性的工业过程迈进,研究人员报道了巴斯德毕赤酵母转化为依靠二氧化碳生长的自养生物。通过添加8个异源基因和3个天然基因的缺失,研究人员将巴斯德毕赤酵母的过氧化物酶体甲醇同化途径改造成类似于Calvin–Benson–Bassham循环(主要的天然CO2固定途径)的CO2固定途径。这个菌株能够以0.008 h-1的µmax将CO2作为唯一碳源持续生长。通过适应性实验室进化,特定的生长速率进一步提高至0.018 h-1。这种工程化的巴斯德毕赤酵母菌株可以通过减少温室气体CO2以及避免在食品生产中的其他用途的有机原料的使用来提高可持续性。

据悉,甲基营养型酵母——巴斯德毕赤酵母,被广泛用于工业酶和药物的生产。像大多数生物技术生产宿主一样,巴斯德毕赤酵母是异养的,生长在有机原料上,这些原料在食品和动物饲料的生产中具有竞争性用途。

附:英文原文

Title: The industrial yeast Pichia pastoris is converted from a heterotroph into an autotroph capable of growth on CO 2

Author: Thomas Gassler, Michael Sauer, Brigitte Gasser, Michael Egermeier, Christina Troyer, Tim Causon, Stephan Hann, Diethard Mattanovich, Matthias G. Steiger

Issue&Volume: 2019-12-16

Abstract: The methylotrophic yeast Pichia pastoris is widely used in the manufacture of industrial enzymes and pharmaceuticals. Like most biotechnological production hosts, P. pastoris is heterotrophic and grows on organic feedstocks that have competing uses in the production of food and animal feed. In a step toward more sustainable industrial processes, we describe the conversion of P. pastoris into an autotroph that grows on CO2. By addition of eight heterologous genes and deletion of three native genes, we engineer the peroxisomal methanol-assimilation pathway of P. pastoris into a CO2-fixation pathway resembling the Calvin–Benson–Bassham cycle, the predominant natural CO2-fixation pathway. The resulting strain can grow continuously with CO2 as a sole carbon source at a μmax of 0.008h1. The specific growth rate was further improved to 0.018h1 by adaptive laboratory evolution. This engineered P. pastoris strain may promote sustainability by sequestering the greenhouse gas CO2, and by avoiding consumption of an organic feedstock with alternative uses in food production.

DOI: 10.1038/s41587-019-0363-0

Source: https://www.nature.com/articles/s41587-019-0363-0

期刊信息

Nature Biotechnology:《自然—生物技术》,创刊于1996年。隶属于施普林格·自然出版集团,最新IF:31.864
官方网址:https://www.nature.com/nbt/
投稿链接:https://mts-nbt.nature.com/cgi-bin/main.plex